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Differentially Marked IncP-1β R751 Plasmids for Cloning via Recombineering and Conjugation

We demonstrate here for the first time the use of an IncP-1β plasmid, R751, as a gene capture vehicle for recombineering/conjugation strategies to clone large segments of bacterial genomes (20 – 100 + Kb). We designed R751 derivatives containing alternative markers for greater flexibility when using...

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Publicat a:Pol J Microbiol
Autors principals: BAINS, ASHVEEN, WILSON, JAMES W.
Format: Artigo
Idioma:Inglês
Publicat: Exeley Inc. 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7260700/
https://ncbi.nlm.nih.gov/pubmed/31880899
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.33073/pjm-2019-052
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