Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution

Caenorhabditis elegans (C. elegans) stands out as the only organism in which the challenge of understanding the cellular origins of an entire nervous system can be observed, with single cell resolution, in vivo. Here, we present an integrated protocol for the examination of neurodevelopment in C. el...

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Udgivet i:J Vis Exp
Main Authors: Duncan, Leighton H., Moyle, Mark W., Shao, Lin, Sengupta, Titas, Ikegami, Richard, Kumar, Abhishek, Guo, Min, Christensen, Ryan, Santella, Anthony, Bao, Zhirong, Shroff, Hari, Mohler, William, Colón-Ramos, Daniel A.
Format: Artigo
Sprog:Inglês
Udgivet: 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7255390/
https://ncbi.nlm.nih.gov/pubmed/31233035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59533
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