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GoPrime: Development of an In Silico Framework to Predict the Performance of Real-Time PCR Primers and Probes Using Foot-and-Mouth Disease Virus as a Model
Real-time PCR (rPCR) is a widely accepted diagnostic tool for the detection and quantification of nucleic acid targets. In order for these assays to achieve high sensitivity and specificity, primer and probe-template complementarity is essential; however, mismatches are often unavoidable and can res...
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發表在: | Pathogens |
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Main Authors: | , , , , , |
格式: | Artigo |
語言: | Inglês |
出版: |
MDPI
2020
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7238122/ https://ncbi.nlm.nih.gov/pubmed/32326039 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/pathogens9040303 |
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