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A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

Bacterial canine vector-borne diseases are responsible for some of the most life-threatening conditions of dogs in the tropics and are typically poorly researched with some presenting a zoonotic risk to cohabiting people. Next-generation sequencing based methodologies have been demonstrated to accur...

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Vydáno v:Pathogens
Hlavní autoři: Huggins, Lucas G., Koehler, Anson V., Schunack, Bettina, Inpankaew, Tawin, Traub, Rebecca J.
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7238069/
https://ncbi.nlm.nih.gov/pubmed/32244645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/pathogens9040258
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