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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Every poxvirus mRNA transcribed after viral DNA replication has an evolutionarily conserved, non-templated 5′-poly(A) leader in the 5′-UTR. To dissect the role of 5′-poly(A) leader in mRNA translation during poxvirus infection we developed an in vitro transcribed RNA-based luciferase reporter assay....
Enregistré dans:
| Publié dans: | J Vis Exp |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7236376/ https://ncbi.nlm.nih.gov/pubmed/31107441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59626 |
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