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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Every poxvirus mRNA transcribed after viral DNA replication has an evolutionarily conserved, non-templated 5′-poly(A) leader in the 5′-UTR. To dissect the role of 5′-poly(A) leader in mRNA translation during poxvirus infection we developed an in vitro transcribed RNA-based luciferase reporter assay....

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Podrobná bibliografie
Vydáno v:J Vis Exp
Hlavní autoři: Dhungel, Pragyesh, Cantu, Fernando, Hernandez, Candy, Yang, Zhilong
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7236376/
https://ncbi.nlm.nih.gov/pubmed/31107441
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59626
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