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From sequence to function: a new workflow for nitrilase identification
ABSTRACT: Nitrilases are industrially important biocatalysts due to their ability to degrade nitriles to carboxylic acids and ammonia. In this study, a workflow for simple and fast recovery of nitrilase candidates from metagenomes is presented. For identification of active enzymes, a NADH-coupled hi...
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| Udgivet i: | Appl Microbiol Biotechnol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Springer Berlin Heidelberg
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7228900/ https://ncbi.nlm.nih.gov/pubmed/32291488 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-020-10544-9 |
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