TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii

Generation and subsequent analysis of mutants is critical to understanding the functions of genes and proteins. Here we describe TIM, an efficient, cost-effective, CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomonas reinhardtii. TIM utilizes delivery into the ce...

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Publicat a:PLoS One
Autors principals: Picariello, Tyler, Hou, Yuqing, Kubo, Tomohiro, McNeill, Nathan A., Yanagisawa, Haru-aki, Oda, Toshiyuki, Witman, George B.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2020
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7219734/
https://ncbi.nlm.nih.gov/pubmed/32401787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0232594
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