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Microwaving and fluorophore-tyramide for multiplex immunostaining on mouse adrenals— using un-conjugated primary antibodies from the same host species
Immunostaining is widely used in biomedical research to show the cellular expression pattern of a given protein. Multiplex immunostaining allows labeling using multiple primary antibodies. To minimize antibody cross-reactivity, multiplex immunostaining using indirect staining requires unlabeled prim...
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| Vydáno v: | J Vis Exp |
|---|---|
| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2020
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7216306/ https://ncbi.nlm.nih.gov/pubmed/32150172 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60868 |
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