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Method for measurement of bacillithiol redox potential changes using the Brx-roGFP2 redox biosensor in Staphylococcus aureus

Recent advances in the design of genetically encoded redox biosensors, such as redox-sensitive GFP (roGFP) have facilitated the real-time imaging of the intracellular redox potential in eukaryotic cells at high sensitivity and at spatiotemporal resolution. To increase the specificity of roGFP2 for t...

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Bibliographic Details
Published in:MethodsX
Main Authors: Van Loi, Vu, Antelmann, Haike
Format: Artigo
Language:Inglês
Published: Elsevier 2020
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7214941/
https://ncbi.nlm.nih.gov/pubmed/32420048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2020.100900
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