Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance

We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the fe...

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Udgivet i:PLoS One
Main Authors: Panarelli, E. Gabriele, van der Meer, Harmen, Gast, Peter, Groenen, Edgar J J.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2020
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7213726/
https://ncbi.nlm.nih.gov/pubmed/32392255
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0232555
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