Chemical roadblocking of DNA transcription for nascent RNA display

Site-specific arrest of RNA polymerases (RNAPs) is fundamental to several technologies that assess RNA structure and function. Current in vitro transcription “roadblocking” approaches inhibit transcription elongation by blocking RNAP with a protein bound to the DNA template. One limitation of protei...

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Bibliographic Details
Published in:J Biol Chem
Main Authors: Strobel, Eric J., Lis, John T., Lucks, Julius B.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2020
Subjects:
Methods and Resources
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7212663/
https://ncbi.nlm.nih.gov/pubmed/32209658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.012641
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