Total Protein Staining is Superior to Classical or Tissue-Specific Protein Staining for Standardization of Protein Biomarkers in Heterogeneous Tissue Samples

Protein detection techniques such as western blotting and ELISA rely on housekeeping proteins as standards for sample normalization. However, clinical or animal tissue specimens are heterogeneous due to presence of contaminating cell types and tissues (e.g., blood vessels and muscle) or cellular dec...

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Veröffentlicht in:Gene Rep
Hauptverfasser: Bettencourt, Jacob W., McLaury, Alex R., Limberg, Afton K., Vargas-Hernandez, Juan S., Bayram, Banu, Owen, Aaron R., Berry, Daniel J., Sanchez-Sotelo, Joaquin, Morrey, Mark E., van Wijnen, Andre J., Abdel, Matthew P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2020
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7202473/
https://ncbi.nlm.nih.gov/pubmed/32377595
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.genrep.2020.100641
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