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Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale

Cultivation of yeast Pichia pastoris in the microtiter plate, for optimisation of culture conditions, and expression screening of transformants has gained significance in recent years. However, in the microtiter plate, it has been challenging to attain cell densities similar to well-aerated shake-fl...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Kaushik, Neha, Lamminmäki, Urpo, Khanna, Navin, Batra, Gaurav
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7198582/
https://ncbi.nlm.nih.gov/pubmed/32366873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-63995-5
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