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Flow Cytometric Quantification of Fatty Acid Uptake byMycobacterium tuberculosis in Macrophages

Mycobacterium tuberculosis (Mtb) has evolved to assimilate fatty acids from its host. However, until recently, there was no reliable way to quantify fatty acid uptake by the bacteria during host cell infection. Here we describe a new method to quantify fatty acid uptake by intracellular bacilli. We...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Nazarova, Evgeniya V., Podinovskaia, Maria, Russell, David G., VanderVen, Brian C.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7198053/
https://ncbi.nlm.nih.gov/pubmed/32368567
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2734
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