Expansion microscopy of C. elegans

We recently developed expansion microscopy (ExM), which achieves nanoscale-precise imaging of specimens at ~70 nm resolution (with ~4.5x linear expansion) by isotropic swelling of chemically processed, hydrogel-embedded tissue. ExM of C. elegans is challenged by its cuticle, which is stiff and imper...

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Bibliographic Details
Published in:eLife
Main Authors: Yu, Chih-Chieh (Jay), Barry, Nicholas C, Wassie, Asmamaw T, Sinha, Anubhav, Bhattacharya, Abhishek, Asano, Shoh, Zhang, Chi, Chen, Fei, Hobert, Oliver, Goodman, Miriam B, Haspel, Gal, Boyden, Edward S
Format: Artigo
Language:Inglês
Published: eLife Sciences Publications, Ltd 2020
Subjects:
Developmental Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7195193/
https://ncbi.nlm.nih.gov/pubmed/32356725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.46249
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