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Selection and exploitation of prevalent, tandemly repeated genomic targets for improved real-time PCR-based detection of Wuchereria bancrofti and Plasmodium falciparum in mosquitoes

BACKGROUND: Optimization of polymerase chain reaction (PCR)-based diagnostics requires the careful selection of molecular targets that are both highly repetitive and pathogen-specific. Advances in both next-generation sequencing (NGS) technologies and bioinformatics-based analysis tools are facilita...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Zulch, Michael F., Pilotte, Nils, Grant, Jessica R., Minetti, Corrado, Reimer, Lisa J., Williams, Steven A.
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7194414/
https://ncbi.nlm.nih.gov/pubmed/32357154
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0232325
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