RNase H-dependent PCR-enabled T Cell Receptor sequencing (rhTCRseq) for Highly Specific and Efficient Targeted Sequencing of T Cell Receptor mRNA for Single-Cell and Repertoire Analysis

rhTCRseq (RNase H-dependent PCR-enabled T cell receptor sequencing) is a procedure that can be used to determine paired alpha/beta T cell receptor (TCR) clonotypes in single cells or perform both alpha and beta TCR repertoire analysis in bulk RNA samples. Relying on the enhanced specificity of RNase...

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Publicado no:Nat Protoc
Main Authors: Li, Shuqiang, Sun, Jing, Allesøe, Rosa, Datta, Krishnalekha, Bao, Yun, Oliveira, Giacomo, Forman, Juliet, Jin, Roger, Olsen, Lars Rønn, Keskin, Derin B., Shukla, Sachet A., Wu, Catherine J., Livak, Kenneth J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7189368/
https://ncbi.nlm.nih.gov/pubmed/31341290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0195-x
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