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Single cell chromatin immunocleavage sequencing (scChIC-Seq) to profile histone modification
We report a single-cell chromatin immunocleavage sequencing (scChIC-seq) methodology for analyzing histone modifications, which involves targeting of the micrococcal nuclease (MNase) by tethering it to an antibody and selective PCR amplification of cleaved target sites. We show that the protocol rel...
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| Publicado no: | Nat Methods |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7187538/ https://ncbi.nlm.nih.gov/pubmed/30923384 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0361-7 |
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