Preparative and Kinetic Analysis of β‐1,4‐ and β‐1,3‐Glucan Phosphorylases Informs Access to Human Milk Oligosaccharide Fragments and Analogues Thereof

The enzymatic synthesis of oligosaccharides depends on the availability of suitable enzymes, which remains a limitation. Without recourse to enzyme engineering or evolution approaches, herein we demonstrate the ability of wild‐type cellodextrin phosphorylase (CDP: β‐1,4‐glucan linkage‐dependent) and...

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Detalhes bibliográficos
Publicado no:Chembiochem
Main Authors: Singh, Ravindra Pal, Pergolizzi, Giulia, Nepogodiev, Sergey A., de Andrade, Peterson, Kuhaudomlarp, Sakonwan, Field, Robert A.
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7187349/
https://ncbi.nlm.nih.gov/pubmed/31657512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201900440
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