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Glycosynthase reaction meets the flow: Continuous synthesis of lacto‐N‐triose II by engineered β‐hexosaminidase immobilized on solid support
The D746E variant of Bifidobacterium bifidum β‐N‐acetyl‐hexosaminidase is a promising glycosynthase (engineered glycosidase deficient in hydrolase activity) for the synthesis of lacto‐N‐triose II (LNT II), a core structural unit of human milk oligosaccharides. Here, we develop a flow process for the...
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| Udgivet i: | Biotechnol Bioeng |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
John Wiley and Sons Inc.
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7187300/ https://ncbi.nlm.nih.gov/pubmed/32017022 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.27293 |
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