Thioguanosine Conversion Enables mRNA‐Lifetime Evaluation by RNA Sequencing Using Double Metabolic Labeling (TUC‐seq DUAL)

Temporal information about cellular RNA populations is essential to understand the functional roles of RNA. We have developed the hydrazine/NH(4)Cl/OsO(4)‐based conversion of 6‐thioguanosine (6sG) into A′, where A′ constitutes a 6‐hydrazino purine derivative. A′ retains the Watson–Crick base‐pair mo...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Gasser, Catherina, Delazer, Isabel, Neuner, Eva, Pascher, Katharina, Brillet, Karl, Klotz, Sarah, Trixl, Lukas, Himmelstoß, Maximilian, Ennifar, Eric, Rieder, Dietmar, Lusser, Alexandra, Micura, Ronald
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7186826/
https://ncbi.nlm.nih.gov/pubmed/31999864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201916272
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