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One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses
A one-tube reverse transcription-polymerase chain reaction (RT-PCR) for absolute feline coronavirus (FCoV) quantitation was developed. The assay is based on the 5′ nuclease activity of the Thermus flavus (Tfl) polymerase and a fluorogenic probe which generates fluorescence when it is cleaved. The fl...
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| izdano v: | J Virol Methods |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier Science B.V.
1999
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7185542/ https://ncbi.nlm.nih.gov/pubmed/10029323 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(98)00129-3 |
| Oznake: |
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