Keth-seq for transcriptome-wide RNA structure mapping

RNA secondary structure is critical to RNA regulation and function. We report a new N(3)-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N(3)-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-...

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Detalhes bibliográficos
Publicado no:Nat Chem Biol
Main Authors: Weng, Xiaocheng, Gong, Jing, Chen, Yi, Wu, Tong, Wang, Fang, Yang, Shixi, Yuan, Yushu, Luo, Guanzheng, Chen, Kai, Hu, Lulu, Ma, Honghui, Wang, Pingluan, Zhang, Qiangfeng Cliff, Zhou, Xiang, He, Chuan
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7182492/
https://ncbi.nlm.nih.gov/pubmed/32015521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41589-019-0459-3
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