Construction of Armored RNA Containing Long-Size Chimeric RNA by Increasing the Number and Affinity of the Pac Site in Exogenous RNA and Sequence Coding Coat Protein of the MS2 Bacteriophage

OBJECTIVES: To construct a one-plasmid expression system of the armored RNA containing long chimeric RNA byincreasing the number and affinity of the pac site. METHODS: The plasmid pET-MS2-pac was constructedwith one C-variant pac site, and then the plasmid pM-CR-2C containing 1,891-bp chimeric seque...

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Vydáno v:Intervirology
Hlavní autoři: Wei, Baojun, Wei, Yuxiang, Zhang, Kuo, Yang, Changmei, Wang, Jing, Xu, Ruihuan, Zhan, Sien, Lin, Guigao, Wang, Wei, Liu, Min, Wang, Lunan, Zhang, Rui, Li, Jinming
Médium: Artigo
Jazyk:Inglês
Vydáno: S. Karger AG 2008
Témata:
Original Paper
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7179527/
https://ncbi.nlm.nih.gov/pubmed/18594159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1159/000141707
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