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Expression and partial purification of recombinant tomato ringspot nepovirus 3C-like proteinase: comparison of the activity of the mature proteinase and the VPg-proteinase precursor

The 3C-like proteinase (Pro) from Tomato ringspot virus (genus Nepovirus) is responsible for the processing of the RNA1-encoded (P1) and RNA2-encoded (P2) polyproteins. Cleavage between the VPg and Pro domains is inefficient in vitro and in E. coli, resulting in the accumulation of the VPg-Pro. In t...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Virus Res
Prif Awduron: Chisholm, Joan, Wieczorek, Andrew, Sanfaçon, Hélène
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Elsevier Science B.V. 2001
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7172257/
https://ncbi.nlm.nih.gov/pubmed/11551656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0168-1702(01)00344-6
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