Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples

In this study, we describe a duplex real-time PCR assay for the simultaneous detection of KIPyV and WUPyV polyomaviruses based on TaqMan probes. This assay detected 500 copies/mL both for KIPyV and WUPyV in 100% of tested positive samples. We assessed this technique on 482 nasopharyngeal aspirate sp...

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Bibliographic Details
Published in:Mol Cell Probes
Main Authors: Ligozzi, Marco, Galia, Liliana, Carelli, Maria, Piccaluga, Pier Paolo, Diani, Erica, Gibellini, Davide
Format: Artigo
Language:Inglês
Published: Published by Elsevier Ltd. 2018
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7172048/
https://ncbi.nlm.nih.gov/pubmed/29883628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2018.06.001
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