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Natural Killer Cell Functions and Subsets After In Vitro Stimulation with IL‐2 and IL‐12, with Special Emphasis on Intracellular IFN‐γ and NK‐Cell Cytotoxicity

Materials and methods:  Isolated cryopreserved human peripheral blood mononuclear cells (PBMCs) were stimulated with IL‐2 and IL‐12. This stimulation has previously been shown to activate NK cells. Cell cytotoxicity was measured by flow cytometry after incubation with k562 cells. This method was com...

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Detalhes bibliográficos
Publicado no:Scand J Immunol
Main Authors: Nyboe, R., Rix, T., Krog, J., Tønnesen, E., Hokland, M.
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Science Ltd/Inc. 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7169545/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.0300-9475.2004.01423y.x
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