Improved detection of rhinoviruses in nasal and throat swabs by seminested RT‐PCR

A seminested RT‐PCR (nRT‐PCR) was used to detect picornavirus (PV) RNA in cell cultures inoculated with rhinoviruses (HRVs) and enteroviruses (EVs). PCR tests in which a primary "touchdown" PCR was followed by secondary reactions using PV or HRV specific primers were able to differentiate...

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Bibliographic Details
Published in:J Med Virol
Main Authors: Ireland, D. C., Kent, J., Nicholson, K. G.
Format: Artigo
Language:Inglês
Published: Wiley Subscription Services, Inc., A Wiley Company 2005
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Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7166614/
https://ncbi.nlm.nih.gov/pubmed/8395557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jmv.1890400204
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