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Reverse transcription polymerase chain reaction (RT‐PCR) for diagnosis of respiratory syncytial virus infection in adults: Use of a single‐tube “hanging droplet” nested PCR

Rapid diagnosis of Respiratory Syncytial virus (RSV) infection is difficult in elderly persons due to the low quantities of virus shed. Therefore, reverse transcription‐polymerase chain reaction (RT‐PCR) was used to detect viral RNA in respiratory secretions. A single‐tube nested RT‐PCR that used pr...

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Détails bibliographiques
Publié dans:J Med Virol
Auteurs principaux: Walsh, Edward E., Falsey, Ann R., Swinburne, Ian A., Formica, Maria A.
Format: Artigo
Langue:Inglês
Publié: John Wiley & Sons, Inc. 2001
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7166324/
https://ncbi.nlm.nih.gov/pubmed/11170067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1096-9071(200103)63:3<259::AID-JMV1010>3.0.CO;2-X
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