Serum‐free transient protein production system based on adenoviral vector and PER.C6 technology: High yield and preserved bioactivity

Stable E1 transformed cells, like PER.C6, are able to grow at scale and to high cell densities. E1‐deleted adenoviruses replicate to high titer in PER.C6 cells whereas subsequent deletion of E2A from the vector results in absence of replication in PER.C6 cells and drastically lowers the expression o...

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Publicado no:Biotechnol Bioeng
Main Authors: Havenga, M.J.E., Holterman, L., Melis, I., Smits, S., Kaspers, J., Heemskerk, E., van der Vlugt, R., Koldijk, M., Schouten, G.J., Hateboer, G., Brouwer, K., Vogels, R., Goudsmit, J.
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2007
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Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7161845/
https://ncbi.nlm.nih.gov/pubmed/18512821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.21757
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