Generating homogenous cortical preplate and deep-layer neurons using a combination of 2D and 3D differentiation cultures

Embryonic stem cells (ESCs) can be used to derive different neural subtypes. Current differentiation protocols generate heterogeneous neural subtypes rather than a specific neuronal population. Here, we present a protocol to derive separate two-deep layer cortical neurons from mouse ESCs (mESCs). mE...

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Vydáno v:Sci Rep
Hlavní autoři: Alsanie, Walaa F., Bahri, Ola A., Habeeballah, Hamza H., Alhomrani, Majid, Almehmadi, Mazen M., Alsharif, Khalaf, Felemban, Ebaa M., Althobaiti, Yusuf S., Almalki, Atiah H., Alsaab, Hashem O., Gaber, Ahmed, Hassan, Mohamed M., Hardy, Ana Maria Gregio, Alhadidi, Qasim
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2020
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7156727/
https://ncbi.nlm.nih.gov/pubmed/32286346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-62925-9
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