A real-time PCR assay to accurately quantify polar bear DNA in fecal extracts

DNA extracted from fecal samples contains DNA from the focal species, food, bacteria and pathogens. Most DNA quantification methods measure total DNA and cannot differentiate among sources. Despite the desirability of noninvasive fecal sampling for studying wildlife populations, low amounts of focal...

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Detalles Bibliográficos
Publicado en:PeerJ
Autores principales: Hayward, Kristen M., Harwood, Michelle P., Lougheed, Stephen C., Sun, Zhengxin, Van Coeverden de Groot, Peter, Jensen, Evelyn L.
Formato: Artigo
Lenguaje:Inglês
Publicado: PeerJ Inc. 2020
Materias:
Biochemistry
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7147431/
https://ncbi.nlm.nih.gov/pubmed/32292653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.8884
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