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Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells

The apoptotic protease-activating factor 1 (Apaf-1) split luciferase biosensor has been used as a biological tool for the detection of early stage of apoptosis. The effect of doxorubicin in a cell-based assay and the addition of cytochrome c and ATP in a cell-free system have been used to test the f...

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Publicado en:Sensors (Basel)
Autores principales: Oladzad, Azarakhsh, Nikkhah, Maryam, Hosseinkhani, Saman
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7146213/
https://ncbi.nlm.nih.gov/pubmed/32210205
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s20061782
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