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Electroporation and Mass Spectrometry: A New Paradigm for In Situ Analysis of Intact Proteins Direct from Living Yeast Colonies

[Image: see text] Yeasts constitute an oft-neglected class of pathogens among which the resistance to first-line treatments, attributed in part to mutations in efflux pumps, is rapidly emerging. Their thick, chitin-reinforced cell walls render cell lysis difficult, complicating their analysis and id...

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Veröffentlicht in:Anal Chem
Hauptverfasser: Kocurek, Klaudia I., Havlikova, Jana, Buchan, Emma, Tanner, Andrew, May, Robin C., Cooper, Helen J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Chemical Society 2020
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7145282/
https://ncbi.nlm.nih.gov/pubmed/31922714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b04365
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