Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

Whereas 16S rRNA gene amplicon sequencing quantifies relative abundances of bacterial taxa, variation in total bacterial load between samples restricts its ability to reflect absolute concentrations of individual bacterial species. Quantitative PCR (qPCR) can quantify individual species, but it is n...

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Vydáno v:mSystems
Hlavní autoři: Tettamanti Boshier, Florencia A., Srinivasan, Sujatha, Lopez, Anthony, Hoffman, Noah G., Proll, Sean, Fredricks, David N., Schiffer, Joshua T.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2020
Témata:
Methods and Protocols
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7141891/
https://ncbi.nlm.nih.gov/pubmed/32265316
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSystems.00777-19
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