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Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation

We report the construction of a single-component optogenetic Rac1 (opto-Rac1) to control actin polymerization by dynamic membrane recruitment. Opto-Rac1 is a fusion of wildtype human Rac1 small GTPase to the C-terminal region of BcLOV4, a LOV (light-oxygen-voltage) photoreceptor that rapidly binds t...

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Detalhes bibliográficos
Publicado no:Photochem Photobiol Sci
Main Authors: Berlew, Erin E., Kuznetsov, Ivan A., Yamada, Keisuke, Bugaj, Lukasz J., Chow, Brian Y.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7141788/
https://ncbi.nlm.nih.gov/pubmed/32048687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9pp00434c
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