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Chromatographic separation of PTAD-derivatized 25-hydroxyvitamin D(3) and its C-3 epimer from human serum and murine skin

The detection of 25-hydroxyvitamin D at low levels in biological samples is facilitated by the use of chemical derivatization with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) in concert with liquid chromatography–tandem mass spectrometry (LC–MS/MS). This mode of analysis is notably hampered by chroma...

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Detalhes bibliográficos
Publicado no:J Chromatogr B Analyt Technol Biomed Life Sci
Main Authors: Teegarden, Matthew D., Riedl, Kenneth M., Schwartz, Steven J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7141139/
https://ncbi.nlm.nih.gov/pubmed/25955382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2015.04.011
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