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Measurement of the concentration of murine IgG monoclonal antibody in hybridoma supernatants and ascites in absolute units by sensitive and reliable enzyme-linked immunosorbent assays (ELISA)

We have investigated an enzyme-linked immunosorbent assay (ELISA) for mouse IgG using affinity-purified goat anti-mouse antibodies for capture and detection. This assay was used to measure the absolute or weight/volume concentration of murine monoclonal antibody in hybridoma supernatants. Bovine or...

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Detalhes bibliográficos
Publicado no:J Immunol Methods
Main Authors: Fleming, J.O., Pen, L.B.
Formato: Artigo
Idioma:Inglês
Publicado em: Published by Elsevier B.V. 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7130540/
https://ncbi.nlm.nih.gov/pubmed/2836513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0022-1759(88)90077-4
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