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Prokaryotic expression, refolding, and purification of fragment 450–650 of the spike protein of SARS-coronavirus
The spike (S) glycoprotein is one of the major structure proteins of SARS-associated coronavirus (CoV). Fragment 450–650 (S450-650) of the S protein contains receptor-binding domain and neutralizing epitopes. In this study, S450-650 was expressed with a histidine tag in Escherichia coli BL21. Bacter...
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| Publicat a: | Protein Expr Purif |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier Inc.
2005
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7129930/ https://ncbi.nlm.nih.gov/pubmed/15642467 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2004.10.004 |
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