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High-level expression of active recombinant ubiquitin carboxyl-terminal hydrolase of Drosophila melanogaster in Pichia pastoris

Ubiquitin carboxyl-terminal hydrolases (UCHs) are implicated in the proteolytic processing of polymeric ubiquitin. The high specificity for the recognition site makes UCHs useful enzymes for in vitro cleavage of ubiquitin fusion proteins. In this work, an active C-terminal His-tagged UCH from Drosop...

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Dades bibliogràfiques
Publicat a:Protein Expr Purif
Autors principals: Jin, Feng-liang, Xu, Xiao-xia, Yu, Xiao-qiang, Ren, Shun-xiang
Format: Artigo
Idioma:Inglês
Publicat: Published by Elsevier Inc. 2009
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7128749/
https://ncbi.nlm.nih.gov/pubmed/18817875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.08.010
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