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Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β
A CHO cell clone (CHO-PoIFN-β) with stable porcine IFN-β expression under control of CMV promoter was selected under G418 pressure. In a 25 cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant PoIFN-β reached 2.3 × 10(6) IU/ml. This cells clone maintained stabl...
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| Publicado no: | Cytokine |
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| Main Authors: | , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Published by Elsevier Ltd.
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7128424/ https://ncbi.nlm.nih.gov/pubmed/21459017 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cyto.2010.12.002 |
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