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Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus
A novel two-step, SYBR Green I based real-time RT-PCR assay was developed for detection and quantification of BCoV using ABI PRISM 7500 sequence detection system. The assay was carried out using two sets of primers designed to amplify highly conserved sequences of the nucleocapsid gene of BCoV and t...
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| Publicat a: | Mol Cell Probes |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier Ltd.
2011
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7127634/ https://ncbi.nlm.nih.gov/pubmed/21419844 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2011.03.001 |
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