Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803, where we aim to target all genes f...

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Gepubliceerd in:Nat Commun
Hoofdauteurs: Yao, Lun, Shabestary, Kiyan, Björk, Sara M., Asplund-Samuelsson, Johannes, Joensson, Haakan N., Jahn, Michael, Hudson, Elton P.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2020
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7125299/
https://ncbi.nlm.nih.gov/pubmed/32245970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-15491-7
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