Improving Identification of In-organello Protein-Protein Interactions Using an Affinity-enrichable, Isotopically Coded, and Mass Spectrometry-cleavable Chemical Crosslinker

An experimental and computational approach for identification of protein-protein interactions by ex vivo chemical crosslinking and mass spectrometry (CLMS) has been developed that takes advantage of the specific characteristics of cyanurbiotindipropionylsuccinimide (CBDPS), an affinity-tagged isotop...

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Detalles Bibliográficos
Publicado en:Mol Cell Proteomics
Main Authors: Makepeace, Karl A. T., Mohammed, Yassene, Rudashevskaya, Elena L., Petrotchenko, Evgeniy V., Vögtle, F.-Nora, Meisinger, Chris, Sickmann, Albert, Borchers, Christoph H.
Formato: Artigo
Idioma:Inglês
Publicado: The American Society for Biochemistry and Molecular Biology 2020
Assuntos:
Research
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7124466/
https://ncbi.nlm.nih.gov/pubmed/32051233
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.RA119.001839
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