Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization

We have developed a sensitive method for the detection of specific genes simultaneously. First, DNA was amplified by a novel asymmetric multiplex PCR with universal primer(s). Second, the 6-carboxytetramethylrhodamine (TAMRA)-labeled PCR products were hybridized specifically with oligonucleotide mic...

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Vydáno v:Microarrays in Clinical Diagnostics
Hlavní autoři: Zhang, Zhi-wei, Zhou, Yi-ming, Zhang, Yan, Guo, Yong, Tao, Sheng-ce, Li, Ze, Zhang, Qiong, Cheng, Jing
Médium: Artigo
Jazyk:Inglês
Vydáno: 2005
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7122606/
https://ncbi.nlm.nih.gov/pubmed/16156097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1385/1-59259-923-0:59
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