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Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction

An RT-PCR-hybridization was developed that amplified genetic material from the M protein gene of HCoV-229E and HCoV-OC43. The analytic sensitivity of these original primers were compared with primers defined in the N gene and described previously. The results show that 0.05 TCID(50) of HCoV-229E and...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Vabret, Astrid, Mouthon, Franck, Mourez, Thomas, Gouarin, Stephanie, Petitjean, Joëlle, Freymuth, François
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Science B.V. 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7119936/
https://ncbi.nlm.nih.gov/pubmed/11483217
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0166-0934(01)00343-3
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