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Quantitative real-time PCR for the measurement of feline cytokine mRNA
We have developed real-time PCR systems to quantitate feline cytokine gene expression. The method is based on the cleavage of fluorescent dye-labelled probes by the 5′–3′ exonuclease activity of the Taq DNA polymerase during PCR and measurement of fluorescence intensity by a Sequence Detection Syste...
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| Publicado no: | Vet Immunol Immunopathol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier Science B.V.
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7119904/ https://ncbi.nlm.nih.gov/pubmed/10587308 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0165-2427(99)00100-2 |
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