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Polymerase chain reaction for probe synthesis and for direct amplification in detection of bovine coronavirus
The polymerase chain reaction (PCR) was used to synthesize ds and ss probes for the detection of bovine coronavirus (BCV) using recombinant plasmids as template molecules. The ds probes detected a minimum of about 2 × 10(5) viral genomes after exposure for 1 h, a detection limit similar to nick-tran...
में बचाया:
| में प्रकाशित: | J Virol Methods |
|---|---|
| मुख्य लेखकों: | , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
Published by Elsevier B.V.
1990
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7119836/ https://ncbi.nlm.nih.gov/pubmed/2266145 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0166-0934(90)90052-H |
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