Multiplex semi-nested RT-PCR with exogenous internal control for simultaneous detection of bovine coronavirus and group A rotavirus

Neonatal calf diarrhea is a multi-etiology syndrome of cattle and direct detection of the two major agents of the syndrome, group A rotavirus and Bovine coronavirus (BCoV) is hampered by their fastidious growth in cell culture. This study aimed at developing a multiplex semi-nested RT-PCR for simult...

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Bibliographische Detailangaben
Veröffentlicht in:J Virol Methods
Hauptverfasser: Asano, Karen Miyuki, de Souza, Sibele Pinheiro, de Barros, Iracema Nunes, Ayres, Giselle Razera, Silva, Sheila Oliveira Souza, Richtzenhain, Leonardo José, Brandão, Paulo E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier B.V. 2010
Schlagworte:
Protocols
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7119652/
https://ncbi.nlm.nih.gov/pubmed/20723564
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2010.08.008
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