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Optimization of extraction and PCR amplification of RNA extracts from paraffin-embedded tissue in different fixatives

A method was developed for fast and efficient isolation of RNA from paraffin-embedded tissue sections for subsequent PCR analysis. This method is based on the binding of RNA to acid-treated glass beads in the presence of a high molarity of guanidinium salt. It can be completed within an hour, and ob...

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Foilsithe in:J Virol Methods
Main Authors: Koopmans, Marion, Monroe, Stephan S., Coffield, Lisa M., Zaki, Sherif R.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Published by Elsevier B.V. 1993
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7119522/
https://ncbi.nlm.nih.gov/pubmed/8396155
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0166-0934(93)90076-4
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