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Optimization of extraction and PCR amplification of RNA extracts from paraffin-embedded tissue in different fixatives
A method was developed for fast and efficient isolation of RNA from paraffin-embedded tissue sections for subsequent PCR analysis. This method is based on the binding of RNA to acid-treated glass beads in the presence of a high molarity of guanidinium salt. It can be completed within an hour, and ob...
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| Published in: | J Virol Methods |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Published by Elsevier B.V.
1993
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7119522/ https://ncbi.nlm.nih.gov/pubmed/8396155 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0166-0934(93)90076-4 |
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